Dave King parked his tractor where the work was supposed to take place for a couple of hours before he said he was moved on by the police.
Mr King said it was a mistake to close the intersection.
“It's a vital part of local traffic and it will cause more dangers than it prevents by closing this road.”
Community member Craig Baldwin said he felt RRV didn't consider other alternative options, and that nearby landowners were frustrated by a "lack of consultancy".
“First we heard about it was on February 19 when they did a letter drop saying they were going to close it in March; so we've been able to hold them off,” he said.
Local Tony Cardamone said many trucks and locals would be forced to use the Hawdon Rd and Murray Valley Hwy intersection up the road, which he believed would become more dangerous due to a higher volume of traffic.
A state Department of Transport spokesperson previously said the works would remove previously identified safety issues faced by drivers at the intersection.
“Safety improvements at the high-risk intersection of Hawdon Rd and Trevaskis Rd will make it safer for all users,” the spokesperson said.
“Closing access to Trevaskis Rd at Hawdon Rd is the safest option for this intersection, which has a history of accidents, including two people tragically losing their lives (in) recent years.”
In late February about 40 people from Wyuna and its surrounds protested at the intersection soon after the announcement came.
In March, Victorian Roads Minister Ben Carroll wrote to State Member for Murray Plains Peter Walsh stating he understood the negative response to the decision but the works were being done in response to eight serious traffic incidents at the intersection in the past 10 years.