Melbourne artist Jimmy D'Vate began priming the silo on Tuesday, and plans to complete his painting by mid-December.
The design will remain a secret but is inspired from the flora and fauna of the Barmah Forest, following community consultation.
Picola and District Improvement Group president Jeanette Holland said the work was part of a two stage project.
“We've got a metal silo that is being painted and a concrete silo that we would also like to paint,” Ms Holland said.
“There’s been lots of organising getting ready for the artist to start, because we've had to get the lifting equipment.
“Our local hardware shop, Limos Home Hardware, has been extremely helpful in getting the paint for us.”
She said she was confident the silo art would bring more people through Picola.
“It’s taken us a long time to get to this stage and I think it will be a boost to the community,” she said.
Mr D'Vate was ready to commence painting back in August but was forced to remain in lockdown in Melbourne due to the coronavirus pandemic.
“It's been a long process and a long wait and it's nice to finally be able to start,” he said.
“It's been really hard, most of my work is based regionally, so since March I haven't been able to do much outside of Melbourne.”
Mr D'Vate will be working on the project for about four weeks.
“I'm really looking forward to it and anyone who wants to come have a look is welcome.”
The artwork was funded by a $53,100 Victorian Government Creative Activations Fund grant, along with money raised through the local community, including the Moira Shire Council, Western Moira Tourism and Bendigo Bank.