The new virus cases emerged after 32,506 tests across Queensland in the 24 hours to 6.30am on Tuesday.
There are 819 patients being treated for COVID-19 in hospital and another 50 in intensive care with 18 people on ventilators.
Health Minister Yvette D'Ath said only one of the 45 virus deaths recorded since mid-December had received a booster vaccine.
"I want to emphasise that of these 45 deaths only one person has had a booster shot, and if I can ... for anyone out there to do anything today to prioritise getting your booster, particularly if you are elderly and vulnerable," she told reporters.
"Please come forward and get your booster. We know that it makes a difference."
Ms D'Ath also said that from Tuesday only two visitors will be allowed to visit hospital emergency departments at any time, in a bid to reduce the spread of the virus within and from health facilities.
"We have had some reports from hospital and health services that they're seeing large groups of family members coming into the emergency department together," she said.
"We really need to reduce the number of people coming into our EDS and moving around our wards."
The latest figures show that 91.59 per cent of eligible Queenslanders have had one dose of a vaccine, while 88.72 per cent have had two.
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