Now-independents Miriam Beswick and Rebekah Pentland were two of three Jacqui Lambie Network candidates voted in at the March state poll.
They were punted from the party by Senator Lambie on Saturday for being "too cosy" with the Liberal minority government and not holding the state infrastructure minister to account.
It is not the first time MPs have left the network - fellow Tasmanian and senator Tammy Tyrrell in March departed saying Senator Lambie was dissatisfied with her performance.
Senator Lambie said she planned to continue running candidates at Senate elections in NSW, Queensland and South Australia.
But she said the weekend's drama signalled the end of her network running candidates at state level.
"What I've done is try to get ordinary Tasmanians into politics," Senator Lambie told ABC radio on Thursday.
"To be honest, that has obviously not worked for whatever reason.
"To ordinary Tasmanians who want a shot in politics now, I am not big enough to make that happen.
"Those days ... of giving ordinary Tasmanians a go are over."
Senator Lambie was elected in 2013 as a candidate for Clive Palmer's party but left the next year and registered her own party in 2015.
She indicated her network did not have the resources to help inexperienced politicians but would again back Andrew Jenner, the sole remaining network MP in Tasmania's parliament, if he wanted to run at the next election.
The network picked up 6.7 per cent of the primary vote at the March Tasmanian election, capitalising on a 12 per cent swing against the Liberal government.
The Liberals were re-elected in minority and signed an agreement with Ms Beswick, Ms Pentland and Mr Jenner, all first-term MPs, for votes of supply and confidence.
Ms Beswick and Ms Pentland on Tuesday inked a fresh deal to support the Liberals, who hold just 14 of 35 lower-house seats.
The pair accused Senator Lambie of not consulting when weighing-in on state issues and not respecting their autonomy.
Mr Jenner said the senator was not difficult to work with but the two had "clashed" on topics.
Ms Beswick, a mother-of-three and small business owner, was elected in the northwest electorate of Braddon, while Bass MP Ms Pentland worked as a pharmaceutical consultant.
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