The group of Jewish activists will gather on the steps of Victorian Parliament on Sunday days after Australian lawmakers passed harsh new penalties aimed at curbing rising anti-Semitic attacks.
Those attacks are "tragic but isolated" and being used to create a narrative that Jews are living in fear, rally organisers say.
"We stand against the genocide in Gaza ... and we reject that the Palestine movement is fermenting anti-Semitism," spokesman David Glanz told AAP.
"We think there's a highly political agenda here, where people claiming to be highly motivated by anti-Semitism are actually motivated to shut down Palestine voices."
Speakers include Jewish comedian Jacob Sacher and barrister Jeffrey Lowenstein, a former chair of the Anti-Defamation Commission whose parents are Holocaust survivors.
Mr Glanz said those who are pro-Israel claim to speak on behalf of all Jews but "there isn't one form of being a Jew".
"Anti-Semitism is real but the politicians and media who put such emphasis on it have nothing to say about the rising tide of hatred faced by our Muslim brothers and sisters," he said.
The rally, to later join up with a weekly Palestine rally, is expected to face a counter-protest from about a dozen Zionists.
The future of Gaza hangs in the balance after US President Donald Trump's plan to clear two million Palestinians from their homeland and resettle them in third countries - ending hope for a two-state solution - was panned by Arab nations and others.
Defence Minister Richard Marles said the occupied territory did not come up during his first meeting with his US counterpart Pete Hegseth on Saturday.
"We did not go through that," he said.
"Our position is really clear, it's what it has always been ... what I'm not about to do is engage in a running commentary on the remarks of the president."
Australia has long maintained its desire for a two-state solution, in which Israel ends its occupation of Gaza and the West Bank and allows the creation of a Palestinian state.
Mr Marles declined to say what Australia would do if Mr Trump sent troops to the occupied territory, which has been decimated since Israel's offensive in response to a major Hamas attack in October 2023.
Mr Trump on Thursday evening walked back talk of US troops entering Gaza while reiterating US would take control of the strip post-war to create his vision of a "Riviera of the Middle East".
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